To stain HUH7 cells with hepcidin using immunohistochemistry
نویسندگان
چکیده
منابع مشابه
Activated macrophages induce hepcidin expression in HuH7 hepatoma cells.
BACKGROUND Hepcidin is an iron regulatory peptide produced by the liver in response to inflammation and elevated systemic iron. Recent studies suggest that circulating monocytes and resident liver macrophages--Küpffer cells--may influence both basal and inflammatory expression of hepcidin. DESIGN AND METHODS We used an in vitro co-culture model to investigate hepatocyte hepcidin regulation in...
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Hepcidin negatively regulates systemic iron homeostasis in response to inflammation and elevated serum iron. Conversely, hepcidin expression is diminished in response to hypoxia, oxidative stress, and increased erythropoietic demand, though the molecular intermediates involved are incompletely understood. To address this, we have investigated hypoxic hepcidin regulation in HuH7 hepatoma cells e...
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ژورنال
عنوان ژورنال: Gut
سال: 2011
ISSN: 0017-5749
DOI: 10.1136/gut.2011.239301.391